Immunoglobulin Y Levels in Egg Yolk From Three Chicken Genotypes
نویسندگان
چکیده
منابع مشابه
Sequential separation of immunoglobulin Y and phosvitin from chicken egg yolk without using organic solvents.
A study was conducted to develop a simple sequential separation protocol to separate phosvitin and IgY from egg yolk without using organic solvents. Egg yolk was diluted with 2 volumes of distilled water (DW), homogenized, and centrifuged. The precipitant was collected and homogenized with 4 volumes of 10% NaCl (wt/vol) in 0.05 N NaOH solution to extract phosvitin. The pH of the homogenate was ...
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background : staphylococcus aureus strains exhibiting multiple antibiotic resistances are isolated from most communities and hospital infections. treatment of patients with these infections has been difficult. the aim of this study was to detect and extract, the egg yolk immunoglobulin y as a potential source of anti- s. aureus antibody. methods : specific igy was produced by immunizing hens wi...
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Chicken egg yolk was found to contain a unique glycosphingolipid pattern not seen in other types of tissue or cell. These glycosphingolipids were isolated in pure form and their structures established by sequential enzymic hydrolysis and permethylation analysis. The major gangliosides in chicken egg yolk are N-acetylneuraminosylgalactosylceramide, N-acetylneuraminosyl-lactosylceramide and di-N-...
متن کاملPreparation of immunoglobulin Y from egg yolk using ammonium sulfate precipitation and ion exchange chromatography.
The objective of this study was to develop an economical, simple, and large-scale separation method for IgY from egg yolk. Egg yolk diluted with 9 volumes of cold water was centrifuged after adjusting the pH to 5.0. The supernatant was added with 0.01% charcoal or 0.01% carrageenan and centrifuged at 2,800 x g for 30 min. The supernatant was filtered through a Whatman no. 1 filter paper and the...
متن کاملSpecific Chicken Egg Yolk Antibodies against Enterotoxigenic Escherichia coli K99 in Serum and Egg Yolk of Immunized Laying Hens
Enterotoxigenic Escherichia coli K99 is one of the dominant pathogens associated with diarrhea of calves. Immunoglobulin Y (IgY), has been used as an inexpensive alternative to antibiotics for the prevention and therapy of several bacterial infections. The study aimed to prepare IgY antibodies against E. coli K99 and to investigate its in vitro effectiveness. E. c...
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ژورنال
عنوان ژورنال: Food and Agricultural Immunology
سال: 2003
ISSN: 0954-0105,1465-3443
DOI: 10.1080/0954010031000138087